# # The Python Imaging Library. # # SPIDER image file handling # # History: # 2004-08-02 Created BB # # Copyright (c) 2004 by Health Research Inc. (HRI) RENSSELAER, NY 12144. # Copyright (c) 2004 by William Baxter. # Copyright (c) 2004 by Secret Labs AB. # Copyright (c) 2004 by Fredrik Lundh. # ## # Image plugin for the Spider image format. This format is is used # by the SPIDER software, in processing image data from electron # microscopy and tomography. ## # # SpiderImagePlugin.py # # The Spider image format is used by SPIDER software, in processing # image data from electron microscopy and tomography. # # Spider home page: # http://www.wadsworth.org/spider_doc/spider/docs/master.html # # Details about the Spider image format: # http://www.wadsworth.org/spider_doc/spider/docs/image_doc.html # import Image, ImageFile import os, string, struct, sys def isInt(f): try: i = int(f) if f-i == 0: return 1 else: return 0 except: return 0 iforms = [1,3,-11,-12,-21,-22] # There is no magic number to identify Spider files, so just check a # series of header locations to see if they have reasonable values. # Returns no.of bytes in the header, if it is a valid Spider header, # otherwise returns 0 def isSpiderHeader(t): h = (99,) + t # add 1 value so can use spider header index start=1 # header values 1,2,5,12,13,22,23 should be integers if not isInt(h[1]): return 0 if not isInt(h[2]): return 0 if not isInt(h[5]): return 0 if not isInt(h[12]): return 0 if not isInt(h[13]): return 0 if not isInt(h[22]): return 0 if not isInt(h[23]): return 0 # check iform iform = int(h[5]) if not iform in iforms: return 0 # check other header values labrec = int(h[13]) # no. records in file header labbyt = int(h[22]) # total no. of bytes in header lenbyt = int(h[23]) # record length in bytes #print "labrec = %d, labbyt = %d, lenbyt = %d" % (labrec,labbyt,lenbyt) if labbyt != (labrec * lenbyt): return 0 # looks like a valid header return labbyt def isSpiderImage(filename): fp = open(filename,'rb') f = fp.read(92) # read 23 * 4 bytes fp.close() bigendian = 1 t = struct.unpack('>23f',f) # try big-endian first hdrlen = isSpiderHeader(t) if hdrlen == 0: bigendian = 0 t = struct.unpack('<23f',f) # little-endian hdrlen = isSpiderHeader(t) return hdrlen class SpiderImageFile(ImageFile.ImageFile): format = "SPIDER" format_description = "Spider 2D image" def _open(self): # check header n = 23 * 4 # read 23 float values f = self.fp.read(n) try: self.bigendian = 1 t = struct.unpack('>23f',f) # try big-endian first hdrlen = isSpiderHeader(t) if hdrlen == 0: self.bigendian = 0 t = struct.unpack('<23f',f) # little-endian hdrlen = isSpiderHeader(t) if hdrlen == 0: raise SyntaxError, "not a valid Spider file" except struct.error: raise SyntaxError, "not a valid Spider file" # size in pixels (width, height) h = (99,) + t # add 1 value cos' spider header index starts at 1 iform = int(h[5]) if iform != 1: raise SyntaxError, "not a Spider 2D image" self.size = int(h[12]), int(h[2]) if self.bigendian: self.rawmode = "F;32BF" else: self.rawmode = "F;32F" self.mode = "F" self.tile = [("raw", (0, 0) + self.size, hdrlen, (self.rawmode, 0, 1))] # returns a byte image after rescaling to 0..255 def convert2byte(self, depth=255): (min, max) = self.getextrema() m = 1 if max != min: m = depth / (max-min) b = -m * min return self.point(lambda i, m=m, b=b: i * m + b).convert("L") # returns a ImageTk.PhotoImage object, after rescaling to 0..255 def tkPhotoImage(self): import ImageTk return ImageTk.PhotoImage(self.convert2byte(), palette=256) # -------------------------------------------------------------------- # Image series # given a list of filenames, return a list of images def loadImageSeries(filelist=None): " create a list of Image.images for use in montage " if filelist == None or len(filelist) < 1: return imglist = [] for img in filelist: if not os.path.exists(img): print "unable to find %s" % img continue try: im = Image.open(img).convert2byte() except: if not isSpiderImage(img): print img + " is not a Spider image file" continue im.info['filename'] = img imglist.append(im) return imglist # -------------------------------------------------------------------- Image.register_open("SPIDER", SpiderImageFile) if __name__ == "__main__": if not sys.argv[1:]: print "Syntax: python SpiderImagePlugin.py imagefile" sys.exit(1) filename = sys.argv[1] #Image.register_open("SPIDER", SpiderImageFile) im = Image.open(filename) print "image: " + str(im) print "format: " + str(im.format) print "size: " + str(im.size) print "mode: " + str(im.mode) print "max, min: ", print im.getextrema()